Our research aims to contribute to the way the world uses plants to solve problems. Through a combination of proteomics and epigenetic approaches, we are deciphering the molecular mechanisms triggered by adverse environmental conditions. Drought, heat, and pathogens are the main drivers of crop yield losses around the world and their occurrences are projected to increase under the climate change scenario. By gaining insights into how plants respond and adapt to a changing environment, we pave the way for new and better possibilities to improve plant traits needed to meet the increasing demand for plants for food and feed.
Research Interests
Redox signalling to chromatin
Adverse environmental conditions that are the main drivers of oxidative stress in plants trigger extensive transcriptional reprogramming that is partially regulated at the chromatin level which adds critical context for the activity of transcription factors. Chromatin features such as post-translational histone modifications are rapidly altered by histone-modifying enzymes in response to external stimuli. The mechanisms of ROS regulation in the nucleus remain enigmatic despite their profound potential to modulate chromatin remodelling. Our work tries to shed light on how redox regulation impacts the activity and protein interactome of histone-modifying enzymes and genome-wide distribution of histone marks.
Cytokinin - ROS interaction
Plants continuously integrate information about their surroundings into complex signal transduction networks. A plethora of synergistic and antagonistic interactions shape the plant hormonal homeostasis and allow an unprecedented level of flexibility with a limited number of regulatory hubs. Cytokinins, which have been initially believed to be solely developmental players are now gaining attention as important stress modulators. We are studying the interplay between cytokinin and redox signalling.
Funding
- Czech Science Foundation (2022 - 2024) "Redox Signalling to Chromatin in Plants"
- Horizon Europe SE (2023 - 2026) "Stabilizing CROP yield under unfavorable conditions by molecular PRIM(E)ing"
- Internal Grant Agency, MENDELU (2021 - 2022) "Chromatin remodeling during cold acclimation"
- Internal Grant Agency, MENDELU (2022 - 2023) "Knowledge-driven improvement of seaweed-based biostimulants against abiotic stress"
Equipment
Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
Used predominantly for peptide-based mass spectrometry
Q Exactive™ GC Orbitrap™ GC-MS/MS
Used for quantification of primary metabolites and plant hormones